Leucine degradation in cell-free extracts of skeletal muscle

Specificity of the effects of leucine and its metabolites on protein degradation in skeletal muscle.

The effects of leucine, its metabolites, and the 2-oxo acids of valine and isoleucine on protein synthesis and degradation in incubated limb muscles of immature and adult rats were tested. Leucine stimulated protein synthesis but did not reduce proteolysis when leucine transamination was inhibited. 4-Methyl-2-oxopentanoate at concentrations as low as 0.25 mM inhibited protein degradation but di...

Oxidation of leucine by rat skeletal muscle.

ODESSEY, RICHARD, AND ALFRED L. GOLDBERG. Oxidation of leucine by rat skeletal muscle. Am. J. Physiol. 223(6) : 1376-1383. 1972.-In the isolated rat diaphragm, 14C02 production from L-leucine-1 -14C occurred at 86 y0 of the rate of its incorporation into protein. Experiments with L-leucine-U-14C indicate complete oxidation of leucine under these conditions. 14C02 production from leucine-1-14C w...

Protein degradation in human skeletal muscle tissue: the effect of insulin, leucine, amino acids and ions.

1. The protein degradation rate of human skeletal muscle was evaluated in vitro in isolated fibre bundles from the rectus abdominus muscle by measuring the tyrosine released from muscle tissue proteins. Protein metabolism in this semi-intact preparation was compared with that of the intact extensor digitorum longus muscles from rats under the same experimental conditions. 2. Protein balance was...

Does leucine, leucyl-tRNA, or some metabolite of leucine regulate protein synthesis and degradation in skeletal and cardiac muscle?

Role of resistance training with the approach of blood flow restriction in skeletal muscle cell growth

Background: The aim of this study was to investigate the response of ERK1/2 protein and muscular morphological adaptations to a period of resistance training with local blood flow restriction. Materials and methods: Twenty healthy male Wistar rats without clinically evident disease (5 weeks old, 120±7 g weight) were divided into four equal groups: control, control with limited blood flow...